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The operetta-compose package collects Fractal1 tasks to convert and process images from Perkin-Elmer Opera/Operetta high-content microscopes. It includes workflows for drug response profiling built upon the OME-ZARR file standard.

Available Fractal Tasks

Currently the following tasks are part of operetta-compose

Task Description
harmony_to_ome_zarr Convert TIFFs which were exported from Harmony (Operetta/Opera, Perkin-Elmer) to OME-ZARR
stardist_segmentation Segment cells with Stardist
regionprops_measurement Take measurements using regionprops and write the features to the OME-ZARR
label_prediction Make predictions on the selected wells and write them to the OME-ZARR
condition_registration Register the experimental conditions in the OME-ZARR

 


1 Fractal is developed by the UZH BioVisionCenter under the lead of @jluethi under contract with eXact lab S.r.l..